Background:  Human parainfluenzaviruses (HPIV) are enveloped, negative single-stranded RNA viruses (ss(-)RNA) of the family Paramyxoviridae. At present four serotypes are known (types 1, 2, 3, 4). Parainfluenzavirus type 1 and 3 belong to the genus Respirovirus, while parainfluenzavirus type 2 and 4 belong to the genus Rubulavirus. Human parainfluenzavirus type 1 (HPIV-1) is the major cause of acute croup, but can also cause respiratory tract infections at all ages. Human parainfluenzavirus type 2 (HPIV-2) can also cause croup as well as upper and lower respiratory tract illnesses. Human parainfluenzavirus type 3 (HPIV-3) causes bronchiolitis and pneumonia. Human parainfluenzavirus type 4 with the subtypes 4a and 4b is less likely to cause servere disease.

Description:  ViroReal® Kit Parainfluenzavirus uses RT real-time PCR for the detection of the hemagglutinin-neuraminidase glycoprotein gene (HN gene) of human parainfluenzavirus types 1, 2, 3 and 4 (HPIV-1, HPIV-2, HPIV-3, HPIV-4). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well. For more detailed information see "/en/product-overview/">here.

ViroReal® Kit Parainfluenzavirus (IVD)
Order Number Reactions Channel Pathogen Channel IPC Target
DHUV01653 50 FAM, VIC Cy5 HN gene
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: HN gene of Parainfluenzavirus
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • CE-IVD Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: ViroReal® Kit Parainfluenzavirus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.