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Product Manual BactoReal® Kit Trueperella pyogenes Download PDF
Material Safety Data Sheet BactoReal® Kit Trueperella pyogenes Download PDF
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Background:  Trueperella pyogenes (formerly Arcanobacterium pyogenes, Corynebacterium pyogenes, Actinomyces pyogenes) is a Grampositive commensal and opportunistic pathogen of economically important livestock, causing diseases such as mastitis, abscesses and pneumonia.

Description:  BactoReal® Kit Trueperella pyogenes uses real-time PCR for the detection of the 16S rRNA gene of Trueperella pyogenes. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Trueperella pyogenes
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB05113 100 FAM Cy5 16S rRNA
DVEB05153 50 FAM Cy5 16S rRNA
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Trueperella pyogenes
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Trueperella pyogenes is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Trueperella pyogenes
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV910 100 FAM Cy5 16S rRNA
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.