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| Product Manual BactoReal® Taylorella equigenitalis |
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| Material Safety Data Sheet BactoReal® Taylorella equigenitalis |
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Background: T. equigenitalis is a Gram-negative bacterium and the causal agent of contagious equine metritis (CEM). CEM is an inflammation of the endometrium of mares, which usually results in temporary infertility. Taylorella equigenitalis is transmitted mainly during mating. It can also be spread by infected semen during artificial insemination.
Description: BactoReal® Kit Taylorella equigenitalis uses real-time PCR for the detection of the 16S rRNA gene of T. equigenitalis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEB04613 | 100 | FAM | Cy5 | 16S rRNA |
| DVEB04653 | 50 | FAM | Cy5 | 16S rRNA |
- Amplification and detection: 16S rRNA gene of Taylorella equigenitalis
- Real-time PCRwith primern, probes and positive control
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: BactoReal® Kit Taylorella equigenitalis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV510 | 100 | FAM | Cy5 | 16S rRNA |