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Product Manual BactoReal® Taylorella equigenitalis Download PDF
Material Safety Data Sheet BactoReal® Taylorella equigenitalis Download PDF
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Background:  T. equigenitalis is a Gram-negative bacterium and the causal agent of contagious equine metritis (CEM). CEM is an inflammation of the endometrium of mares, which usually results in temporary infertility. Taylorella equigenitalis is transmitted mainly during mating. It can also be spread by infected semen during artificial insemination.

Description:  BactoReal® Kit Taylorella equigenitalis uses real-time PCR for the detection of the 16S rRNA gene of T. equigenitalis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Taylorella equigenitalis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB04613 100 FAM Cy5 16S rRNA
DVEB04653 50 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Taylorella equigenitalis
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Taylorella equigenitalis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Taylorella equigenitalis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV510 100 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The assay detects DNA of Taylorella equigenitalis by targeting the 16S rRNA gene using real-time PCR.
It is the causative agent of contagious equine metritis (CEM), a disease affecting mare fertility.
The assay is validated for DNA extracted from swab samples.
An artificial DNA IPC is added during extraction to verify DNA integrity and detect PCR inhibition. It is detected in the Cy5 channel.
The assay includes only the detection reagents, while the kit provides a complete set including PCR mix and water.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.