Background:  Streptococcus dysgalactiae is a Grampositive bacterium belonging to the group C streptococci. It is known to cause clinically acute and subclinical mastitis in cows, ewes and goats, as well as polyarthritis.

Description:  BactoReal® Kit Streptococcus dysgalactiae uses multiplex real-time PCR for the detection of S. dysgalactiae (S. dysgalactiae subsp. equisimilis and S. dysgalactiae subsp. dysgalactiae). This test is performed in a multiplex real-time PCR format to enable a differentiation between S. dysgalactiae from other Streptococcus species, especially S. canis. It is based on the amplification and detection of the 23S rRNA gene and 16S rRNA gene. In FAM channel the 23S rRNA gene of S. dysgalactiae and S. canis is detected. In VIC/HEX channel the 16S rRNA gene of several Streptococcus species (S. dysgalactiae, S. agalactiae, S. phocae, S. porcinus, S. didelphis) except that of S. canis is detected. Thus, samples showing a positive signal in FAM as well as in VIC/HEX channel are positive for S. dysgalactiae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Streptococcus dysgalactiae
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB01713 100 FAM, VIC Cy5 23S rRNA+16S rRNA
DVEB01753 50 FAM, VIC Cy5 23S rRNA+16S rRNA
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 23S rRNA and 16S rRNA gene of Streptococcus dysgalactiae
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Streptococcus dysgalactiae is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Streptococcus dysgalactiae
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV903 100 FAM, VIC Cy5 23S rRNA+16S rRNA
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.