Background:  The genus Staphylococcus includes more than 50 different species. Staphylococci are commensals on skin and upper respiratory tracts of mammals and birds. However, they can also cause various infections of the skin and a wide variety of diseases in humans and animals through either toxin production or penetration. Another common path of infection is the respiratory tract, where the bacterium causes pneumonia. Staphylococci, mainly Staphylococcus aureus can also cause bovine mastitis.

Description:  BactoReal® Kit Staphylococcus spp. uses real-time PCR for the detection of the 23S rRNA gene of species of the genus Staphylococcus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Staphylococcus spp.
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB03713 100 FAM Cy5 23S rRNA
DVEB03753 50 FAM Cy5 23S rRNA
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 23S rRNA gene of Staphylococcus spp.
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Staphylococcus spp. is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Staphylococcus spp.
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV804 100 FAM Cy5 23S rRNA
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.