Background: Staphylococcus aureus is a Grampositive bacterium. In healthy animals, S. aureus colonization of the skin, intestinal tract, or nasopharynx is generally asymptomatic. However, depending on the strain, S. aureus is capable of secreting several exotoxins. Many of these toxins are associated with specific diseases. In cattle, S. aureus causes one of the most common types of chronic mastitis.

Description: BactoReal® Kit Staphylococcus aureus uses real-time PCR for the detection of the thermonuclease (nuc) gene of S. aureus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

BactoReal® Kit Staphylococcus aureus
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEB01011100FAMVIC/HEXnuc
DVEB01013100FAMCY5nuc
DVEB0105150FAMVIC/HEXnuc
DVEB0105350FAMCY5nuc
For veterinary use only.
Shipping Temperature: +4°C cool packs
Product Features:
  • Amplification and detection: nuc gene of Staphylococcus aureus
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Staphylococcus aureus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.


Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Staphylococcus aureus
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV800100FAM-nuc
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.