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Product Manual BactoReal® Kit Staphylococcus aureus Download PDF
Material Safety Data Sheet BactoReal® Kit Staphylococcus aureus Download PDF
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Background:  Staphylococcus aureus is a Grampositive bacterium. In healthy animals, S. aureus colonization of the skin, intestinal tract, or nasopharynx is generally asymptomatic. However, depending on the strain, S. aureus is capable of secreting several exotoxins. Many of these toxins are associated with specific diseases. In cattle, S. aureus causes one of the most common types of chronic mastitis.

Description:  BactoReal® Kit Staphylococcus aureus uses real-time PCR for the detection of the thermonuclease (nuc) gene of S. aureus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Staphylococcus aureus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB01013 100 FAM Cy5 nuc
DVEB01053 50 FAM Cy5 nuc
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: nuc gene of Staphylococcus aureus
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Staphylococcus aureus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Staphylococcus aureus
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV800 100 FAM Cy5 nuc
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.