Background: Schmallenberg virus (SBV) is a novel Orthobunyavirus that has been associated with disease in ruminants (cattle, sheep and goats) in Europe. It causes transient clinical symptoms in adult cattle and congenital malformation in newborn ruminants. Schmallenberg virus is part of the Simbu serogroup of the Bunyaviridae family, genus Orthobunyavirus. The virus is transmitted via insect vectors, mainly midges.

Description: ViroReal® Kit Schmallenberg Virus uses one-step reverse transcription RT real-time PCR for the detection of the nucleocapsid protein gene of schmallenberg virus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well. For more detailed information see here.

ViroReal® Kit Schmallenberg Virus
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEV03211100FAMVIC/HEXnucleocapsid protein gene
DVEV03213100FAMCY5nucleocapsid protein gene
DVEV0325150FAMVIC/HEXnucleocapsid protein gene
DVEV0325350FAMCY5nucleocapsid protein gene
For veterinary use only.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: nucleocapsid protein gene of Schmallenberg Virus
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit Schmallenberg Virus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Schmallenberg Virus
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV33100FAM-nucleocapsid protein gene
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.