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| Product Manual ViroReal® Schmallenberg Virus |
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| Material Safety Data Sheet ViroReal® Schmallenberg Virus |
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Background: Schmallenberg virus (SBV) is a novel Orthobunyavirus that has been associated with disease in ruminants (cattle, sheep and goats) in Europe. It causes transient clinical symptoms in adult cattle and congenital malformation in newborn ruminants. Schmallenberg virus is part of the Simbu serogroup of the Bunyaviridae family, genus Orthobunyavirus. The virus is transmitted via insect vectors, mainly midges.
Description: ViroReal® Kit Schmallenberg Virus uses one-step reverse transcription RT real-time PCR for the detection of the nucleocapsid protein gene of schmallenberg virus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEV03213 | 100 | FAM | Cy5 | nucleocapsid protein gene |
| DVEV03253 | 50 | FAM | Cy5 | nucleocapsid protein gene |
- Amplification and detection: nucleocapsid protein gene of Schmallenberg Virus
- Real-time PCRwith primern, probes and positive control
- PCR- platforms: runs on all established standard real-time PCR- platforms
PCR platforms: ViroReal® Kit Schmallenberg Virus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV33 | 100 | FAM | Cy5 | nucleocapsid protein gene |