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Product Manual BactoReal® Kit Riemerella anatipestifer Download PDF
Material Safety Data Sheet BactoReal® Kit Riemerella anatipestifer Download PDF
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Background:  Riemerella anatipestifer is a Gram-negative bacterium that causes acute or chronic septicaemia. It primarily affects young ducks and less frequently turkeys and geese throughout the world. There are 21 known serotypes and infection is spread horizontally between birds. Infection may be referred to as Duck Septicaemia, Goose ''flu, Riemerellosis, New Duck Disease and Polyserositis.

Description:  BactoReal® Kit Riemerella anatipestifer uses real-time PCR for the detection of the 16S-23S ribosomal RNA intergenic spacer of Riemerella anatipestifer. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Riemerella anatipestifer
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB05913 100 FAM Cy5 16S-23S ribosomal RNA intergenic spacer
DVEB05953 50 FAM Cy5 16S-23S ribosomal RNA intergenic spacer
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S-23S rRNA spacer of Riemerella anatipestifer
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Riemerella anatipestifer is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Riemerella anatipestifer
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV232 100 FAM Cy5 16S-23S ribosomal RNA intergenic spacer
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.