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Product Manual ViroReal® Rabies Virus Download PDF
Material Safety Data Sheet ViroReal® Rabies Virus Download PDF
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Background:  Rabies is caused by a neurotropic virus of the genus Lyssavirus of the family Rhabdoviridae, and is transmissible to all mammals. As it is transmissible to humans by inoculation or inhalation of infectious virus, all suspected infected material must be handled under the appropriate safety conditions specified by the World Health Organisation (WHO). Previous known genotypes were recently reclassified, and the Lyssavirus genus currently consists of 16 species (Aravan lyssavirus (ARAV), Australian bat lyssavirus (ABLV), Bokeloh bat lyssavirus (BBLV), Duvenhage lyssavirus (DUVV), European bat lyssavirus 1 (EBLV 1), European bat lyssavirus 2 (EBLV 2), Gannoruwa bat lyssavirus (GBLV), Ikoma lyssavirus (IKOV), Irkut lyssavirus (IRKV), Khujand lyssavirus (KHUV), Lagos bat lyssavirus (LBV), Lleida lyssavirus (LLEBV), Mokola lyssavirus (MOKV), Rabies lyssavirus (RABV), Shimoni bat lyssavirus (SHIBV) and the West Caucasian bat lyssavirus (WCBV). All lyssaviruses cause the severe neurotropic disease known as rabies. RABV (genotype 1, RABV-GT-1) is detected worldwide and is the prototype lyssavirus, the remaining species are known as rabies-related lyssaviruses. Human infections are mainly caused by RABV, DUVV, EBLV-1, EBLV-2 and ABLV. ​​​

ViroReal​​®​​ Kit Rabies Virus​ ​​detects the classical RABV genotype 1 and EBLV genotype 5 & 6. It does not detect LBV genotype 2, MOKV genotype 3, DUVV genotype 4 or ABLV genotype 7.

Description:  ViroReal® Kit Rabies Virus uses one-step reverse transcription RT real-time PCR for the detection of the nucleoprotein (N) gene of rabies virus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system.

ViroReal® Kit Rabies Virus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV00813 100 FAM Cy5 nucleoprotein gene
DVEV00853 50 FAM Cy5 nucleoprotein gene
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: N gene of Rabies Virus
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Rabies Virus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

ViroReal® Rabies Virus
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV255 100 FAM Cy5 nucleoprotein gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.