Background: Pseudorabiesvirus (PRV) is a swine DNA herpesvirus of the Alphaherpesvirinae subfamily. It is also known as suid herpesvirus 1 (SHV-1), or by its original name, Aujeszky's disease virus. The pig is the principal reservoir host, but the virus may also infect other mammals like cattle, little ruminants, carnivores and rodents. It is not pathogenic for humans. In swine, Aujeszky’s disease shows itself under three forms: a nervous form, a respiratory form or a genital form. In latently infected pigs, pseudorabiesvirus persists primarily in the trigeminal or sacral ganglia and the tonsils. In other mammals, it causes infection of the central nervous system with neurologic behavioral changes leading very quickly to death.

Description: ViroReal® Kit Pseudorabiesvirus uses real-time PCR for the detection of the glycoprotein B gene of SHV-1. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

ViroReal® Kit Pseudorabiesvirus
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEV02511100FAMVIC/HEXglycoprotein B gene
DVEV02513100FAMCY5glycoprotein B gene
DVEV0255150FAMVIC/HEXglycoprotein B gene
DVEV0255350FAMCY5glycoprotein B gene
For veterinary use only.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: glycoprotein B gene of Pseudorabiesvirus (SHV-1)
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit Pseudorabiesvirusis developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Pseudorabiesvirus
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV27100FAM-glycoprotein B gene
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.