Background: The porcine reproductive and respiratory syndrome virus (PRRSV) is a single strain, positive-sense RNA virus belonging to the order Nidovirales, family Arteriviridae. This virus causes the Porcine Reproductive and Respiratory Syndrome (PRRS). PRRS is one of the most economically important diseases of swines and is also known as Blue-Ear Pig Disease. The disease is characterised by reproduction defects and by multifactor respiratory problems. European (EU) lineage PRRSV isolates (type 1, prototype strain is the lelystad virus) are genetically divergent from North American (US) isolates (type 2, prototype strain is the VR-2332) but cause similar clinical symptoms. A great genetic heterogeneity of field isolates is observed and further complicates diagnosis.

Description: ViroReal® Kit PRRS Virus EU & NA uses one-step reverse transcription RT real-time PCR for the detection of the open-reading frame 7 including the 3'-untranslated region of both viral lineages PRRS virus EU and PRRS virus NA (PRRSV type 1 and PRRSV type 2). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well. For more detailed information see here.

ViroReal® Kit PRRS Virus EU & NA
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEV00113100FAM & VIC/HEXCY5open-reading frame 7
DVEV0015350FAM & VIC/HEXCY5open-reading frame 7
For veterinary use only.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: open-reading frame 7 of PRRSV EU and PRRSV NA
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit PRRS Virus EU & NA is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® PRRS Virus EU & NA
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV1R01100FAM & VIC/HEX-open-reading frame 7
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.