Background:  Porcine hemagglutinating encephalomyelitis virus (PHEV), an RNA virus, is a member of the genus Coronavirus (Betacoronavirus), family Coronaviridae, and primarily infects pigs less than three weeks old. This viral disease of piglets is characterized by vomiting, anorexia and neurological symptoms and results either in rapid death or chronic emaciation (vomiting and wasting). The mortality rate in infected piglets under three weeks old is nearly 100 %, but the incidence of infection is small as most piglets are protected by maternal antibodies. Clinically apparent disease develops only when young piglets from non-immune sows are exposed. The virus is spread via aerosol.

Description:  ViroReal® Kit PHEV uses one-step reverse transcription RT real-time PCR for the detection of the nucleocapsid protein gene (N gene) of the PHEV. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® Kit PHEV
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV01513 100 FAM Cy5 nucleocapsid protein gene
DVEV01553 50 FAM Cy5 nucleocapsid protein gene
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: N gene of PHEV
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit PHEV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® PHEV
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV88 100 FAM Cy5 nucleocapsid protein gene
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.