Background: Porcine epidemic diarrhea virus (PEDV), a member of the genus Coronavirus (Alphacoronavirus), family Coronaviridae, is a positive-sense, enveloped, single-stranded RNA virus. Severity of disease is variable and dependent on the epidemiological status of the herd. When epidemic, PEDV causes acute watery diarrhea and vomiting in swine of all ages. If endemic, then diarrhea is observed with lower morbidity in suckling and recently weaned pigs. The PED virus is similar to, but antigenically distinct from transmissible gastroenteritis virus (TGEV).

Description: ViroReal® Kit PEDV & SDCV uses one-step reverse transcription multiplex RT real-time PCR for the detection of nucleocapsid protein gene (N gene) of PEDV (porcine epidemic diarrhea virus) and of the 3’UTR of SDCV (swine delta coronavirus, PDCoV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well. For more detailed information see here.

ViroReal® Kit PEDV & SDCV
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEV01913100FAM & VIC/HEXCY5nucleocapsid protein gene
DVEV0195350FAM & VIC/HEXCY5nucleocapsid protein gene
For veterinary use only.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: N gene of PEDV and 3’UTR of SDCV
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit PEDV & SDCV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.


Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® PEDV & SDCV
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV69100FAM & VIC/HEX-nucleocapsid protein gene
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.