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Background: Porcine circovirus type 3 (PCV3) is a novel circovirus species identified in pigs with porcine dermatitis and nephropathy syndrome (PDNS), reproductive failure, respiratory disease and multi-systemic inflammation. Porcine circovirus is a small, non-enveloped DNA virus that contains a single strand of circular DNA. It belongs to the genus Circovirus in the family Circoviridae.
Description: ViroReal® Kit PCV3 uses real-time PCR for the detection of capsid gene of the porcine circovirus type 3. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
DVEV03613 | 100 | FAM | Cy5 | capsid gene |
DVEV03653 | 50 | FAM | Cy5 | capsid gene |
- Amplification and detection: capsid gene of PCV3
- Real-time PCRwith primern, probes and positive control
- PCR- platforms: runs on all established standard real-time PCR- platforms
PCR platforms: ViroReal® Kit PCV3 is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
RTGV103 | 100 | FAM | Cy5 | capsid gene |