Background:  Porcine circovirus type 3 (PCV3) is a novel circovirus species identified in pigs with porcine dermatitis and nephropathy syndrome (PDNS), reproductive failure, respiratory disease and multi-systemic inflammation. Porcine circovirus is a small, non-enveloped DNA virus that contains a single strand of circular DNA. It belongs to the genus Circovirus in the family Circoviridae.

Description:  ViroReal® Kit PCV3 uses real-time PCR for the detection of capsid gene of the porcine circovirus type 3. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

ViroReal® PCV3
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV03613 100 FAM Cy5 capsid gene
DVEV03653 50 FAM Cy5 capsid gene
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: capsid gene of PCV3
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit PCV3 is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® PCV3
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV103 100 FAM Cy5 capsid gene
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.