Background:  Porcine cytomegalovirus (PCMV) is a herpesvirus infecting only pigs. PCMV is present worldwide. Most PCMV infections are sub-clinical and clinical disease is rare causing inclusion body rhinitis (IBR), pneumonia, anaemia, fever and abortion or neonatal piglet losses. The virus may also be endemic without causing any apparent clinical disease or economic loss. However, these infected animals can be latent carriers of the virus.

Description:  ViroReal® Kit PCMV uses real-time PCR for the detection of the DNA polymerase (DPOL) gene of the porcine cytomegalovirus (PCMV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit PCMV
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV01113 100 FAM Cy5 DPOL
DVEV01153 50 FAM Cy5 DPOL
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: DPOL gene of PCMV
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms

PCR platforms: ViroReal® Kit PCMV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® PCMV
Order Number Reactions Channel Pathogen Channel IPC Target
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.