Back
Inquiry

Download

Product Manual BactoReal® Kit Ornithobacterium rhinotracheale Download PDF
Material Safety Data Sheet BactoReal® Kit Ornithobacterium rhinotracheale Download PDF
Download All

Background:  Ornithobacterium rhinotracheale (ORT) is a Gram-negative bacterium causing respiratory disease in turkeys, chickens and other avian species. Clinical signs are nasal discharge, sneezing, coughing and sinusitis. Ornithobacterium rhinotracheale is currently the only species within the Ornithobacterium genus and found worldwide.

Description:  BactoReal® Kit Ornithobacterium rhinotracheale uses real-time PCR for the 16S rRNA gene of Ornithobacterium rhinotracheale. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Ornithobacterium rhinotracheale
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB06613 100 FAM Cy5 16S rDNA
DVEB06653 50 FAM Cy5 16S rDNA
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Ornithobacterium rhinotracheale
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Ornithobacterium rhinotracheale is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Ornithobacterium rhinotracheale
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV927 100 FAM Cy5 16S rDNA
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.