Background:  Nocardia is a genus of Grampositive, facultative intracellular bacteria belonging to the Actinomycetes. Nocardia species are found worldwide in soil, water and plants. This genus has 85 species, of these > 25 have been recognized as causes of human infections, and > 30 are responsible for animal diseases. Nocardia spp. cause opportunistic disease in humans and animals. Nocardiosis is an infectious disease affecting either the lungs, but it may spread to other organ systems, most often the brain and the skin. It may also involve the kidneys, joints, heart, eyes, and bones. Bovine mastitis is the most common clinical manifestation of nocardiosis among domestic ruminants. Nocardial mastitis is observed in dairy herds submitted to inadequate milking management and/or poor hygienic conditions in pre- and post-milking. The most important pathogenic species for humans and animals are N. asteroides complex, N. brasiliensis, N. pseudobrasiliensis, N. otitidiscaviarum, and N. transvalensis.

Description:  BactoReal® Kit Nocardia spp. uses real-time PCR for the detection of the 16S rDNA gene of Nocardia spp. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Nocardia spp.
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB03613 100 FAM Cy5 16S rDNA
DVEB03653 50 FAM Cy5 16S rDNA
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rDNA gene of Nocardia spp.
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Nocardia spp. is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Nocardia spp.
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV909 100 FAM Cy5 16S rDNA
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.