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Product Manual ParoReal® Kit Neospora caninum Download PDF
Material Safety Data Sheet ParoReal® Kit Neospora caninum Download PDF
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Background:  Neospora caninum is a coccidian parasite and an important cause of spontaneous abortion in infected livestock. It has a heteroxenous life cycle, with the sexually reproductive stage occurring in the intestine of a definitive host (domestic dog, coyotes, gray wolves and dingos). Intermediate hosts, such as cattle, ingest oocysts passed in the feces of the definitive host. Formation of cysts results in chronic infection of the intermediate host. Neosporosis is an infectious disease for many different canids and cattle. In canines, it leads to neuromuscular degeneration. In dairy and beef cattle, it is an important cause of reproductive problems and abortion. Once the cattle is infected, N. caninum is maintained as a life-long infection and is transmitted from cow to the fetus during pregnancy, resulting in abortion or weak or also normal calves. The occurrence of N. caninum infection in beef and dairy cattle has been reported worldwide.

Description:  ParoReal® Kit Neospora caninum uses real-time PCR for the detection of the the mitochondrial NC5 gene of Neospora caninum. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

ParoReal® Kit Neospora caninum
Order Number Reactions Channel Pathogen Channel IPC Target
DVEP00513 100 FAM Cy5 mitochondrial NC5
DVEP00553 50 FAM Cy5 mitochondrial NC5
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: NC5 gene of Neospora caninum
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ParoReal® Kit Neospora caninum is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ParoReal® Neospora caninum
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV269 100 FAM Cy5 mitochondrial NC5
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.