Background: Avian mycoplasmosis is caused by several pathogenic mycoplasmae. Mycoplasma gallisepticum and Mycoplasma synoviae are the most important avian pathogens. In addition, Mycoplasma meleagridis and Mycoplasma iowae can also cause disease in poultry, but Mycoplasma gallisepticum and Mycoplasma synoviae are considered the main species of pathogenic mycoplasmae. Mycoplasma gallisepticum and Mycoplasma synoviae cause chronic respiratory disease (CRD) and infectious synovitis of chickens and turkeys especially in the presence of stresses or other respiratory pathogens, causing economic losses due to decrease in growth rate, loss of weight and reduced egg production.

Description: BactoReal® Kit Mycoplasma synoviae uses real-time PCR for the detection of the vlhA gene (haemagglutinin encoding gene) gene of M. synoviae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

BactoReal® Kit Mycoplasma synoviae
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEB00211100FAMVIC/HEXvlhA
DVEB00213100FAMCY5vlhA
DVEB0025150FAMVIC/HEXvlhA
DVEB0025350FAMCY5vlhA
For veterinary use only.
Shipping Temperature: +4°C cool packs
Product Features:
  • Amplification and detection: vlhA gene of Mycoplasma synoviae
  • Real-time PCR with primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Mycoplasma synoviae is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.


Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Mycoplasma synoviae
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV907100FAM-vlhA
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.