Background:  Mycoplasma suis (formerly called Eperythrozoon suis) belongs to the hemotrophic mycoplasma group. It is the cause of swine eperythrozoonosis. Acute disease is associated with severe bacteremia and acute hemolytic anemia. In chronically infected pigs clinical signs range from mild icteroanemia, poor growth rates to bad reproductive performance.

Description:  BactoReal® Kit Mycoplasma suis uses real-time PCR for the detection of the 16S rDNA gene of M. suis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Mycoplasma suis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB02613 100 FAM Cy5 16S rDNA
DVEB02653 50 FAM Cy5 16S rDNA
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rDNA gene of Mycoplasma suis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Mycoplasma suis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Mycoplasma suis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV215 100 FAM Cy5 16S rDNA
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.