Background: Members of the Mycobacterium tuberculosis-complex (MTC) cause tuberculosis in animals and humans. The eight closely related species of the MTC have a wide range of natural hosts including bovine hosts (M. bovis), caprine hosts (M. caprae), rodent hosts (M. microti), and pinniped hosts (M. pinnipedii). Bovine tuberculosis remains an important disease in many countries of the world, causing significant economic losses. Infected cattle can excrete the causative agent of tuberculosis via the respiratory tract, faeces or milk depending on the affected organ system.

Description: BactoReal® Kit Mycobacterium tuberculosis complex uses real-time PCR for the ITS2 region of Mycobacterium species belonging to the Mycobacterium tuberculosis complex (M. tuberculosis, M. bovis, M. caprae, M. canetti, M. africanum and M. microti). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

BactoReal® Kit Mycobacterium tuberculosis complex
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEB02711100FAMVIC/HEXITS2 region
DVEB02713100FAMCY5ITS2 region
DVEB0275150FAMVIC/HEXITS2 region
DVEB0275350FAMCY5ITS2 region
For veterinary use only.
Shipping Temperature: +4°C cool packs
Product Features:
  • Amplification and detection: ITS2 region of Mycobacterium tuberculosis complex
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Mycobacterium tuberculosis complex is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Mycobacterium tuberculosis complex
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV250100FAM-ITS2 region
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.