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Product Manual BactoReal® Kit Mycobacterium avium ssp. paratuberculosis Download PDF
Material Safety Data Sheet BactoReal® Kit Mycobacterium avium ssp. paratuberculosis Download PDF
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Background:  Mycobacterium avium ssp. paratuberculosis (MAP) causes Johne''s disease, a chronic gastroenteritis affecting primarily ruminants and many other species. This disease is one of the most widespread and economically important diseases of ruminants. It has a relatively long incubation period characterized by the excretion of MAP in faeces for months and years before first clinical symptoms develop.

Description:  BactoReal® Kit Mycobacterium avium ssp. paratuberculosis uses real-time PCR for the detection of the insertion sequence IS900 of M. avium ssp. paratuberculosis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Mycobacterium avium ssp. paratuberculosis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB02213 100 FAM Cy5 IS900
DVEB02253 50 FAM Cy5 IS900
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: IS900 of Mycobacterium avium ssp. paratuberculosis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Mycobacterium avium ssp. paratuberculosis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Mycobacterium avium ssp. paratuberculosis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV240 100 FAM Cy5 IS900
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.