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Download AllBackground: The Maedi-Visna virus (MVV) also called ovine lentivirus (OvLV) is a single-stranded RNA small ruminant lentivirus (SRLV) within the retrovirus family. There are neurovirulent and non-neurovirulent strains of MVV. The MVV is closely related to the caprine arthritis - encephalitis (CAE) virus. Both infect cells of the monocyte/macrophage lineage and cause lifelong infections. Visna (“wasting”, in Icelandic) is a slow progressive encephalomyelitis of sheep. Although most infections are subclinical, a minority of animals develops progressive, untreatable disease syndromes including dyspnea (maedi), slowly progressive arthritis, mastitis or neurologic signs (visna) such as encephalitis.
Description: ViroReal® Kit Maedi-Visna virus uses one-step reverse transcription RT real-time PCR for the detection of the gag polyprotein gene of Maedi-Visna virus (MVV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well. For more detailed information see here.
| Ordernumber | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEV03511 | 100 | FAM | VIC/HEX | gag polyprotein gene |
| DVEV03513 | 100 | FAM | CY5 | gag polyprotein gene |
| DVEV03551 | 50 | FAM | VIC/HEX | gag polyprotein gene |
| DVEV03553 | 50 | FAM | CY5 | gag polyprotein gene |
Shipping Temperature: -20°C dry ice
- Amplification and detection: gag gene of Maedi-Visna virus
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle RT-PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: ViroReal® Kit Maedi-Visna virus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Ordernumber | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV36 | 100 | FAM | - | gag polyprotein gene |
Shipping Temperature: +4°C cool packs