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Product Manual ViroReal® Maedi-Visna Virus Download PDF
Material Safety Data Sheet ViroReal® Maedi-Visna Virus Download PDF
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Background:  The Maedi-Visna virus (MVV) also called ovine lentivirus (OvLV) is a single-stranded RNA small ruminant lentivirus (SRLV) within the retrovirus family. There are neurovirulent and non-neurovirulent strains of MVV. The MVV is closely related to the caprine arthritis - encephalitis (CAE) virus. Both infect cells of the monocyte/macrophage lineage and cause lifelong infections. Visna (“wasting”, in Icelandic) is a slow progressive encephalomyelitis of sheep. Although most infections are subclinical, a minority of animals develops progressive, untreatable disease syndromes including dyspnea (maedi), slowly progressive arthritis, mastitis or neurologic signs (visna) such as encephalitis.

Description:  ViroReal® Kit Maedi-Visna virus is a real-time PCR for the qualitative detection of the RNA of the gag polyprotein gene of Maedi-Visna virus (MVV). Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit Maedi-Visna Virus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV03513 100 FAM Cy5 gag polyprotein gene
DVEV03553 50 FAM Cy5 gag polyprotein gene
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: RNA of the gag polyprotein of Maedi-Visna virus
  • Transcription with thermostable MMLV Reverse Transcriptase
  • Contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit Maedi-Visna virus was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent) and LightCycler® 480 II (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), cobas z 480 Analyzer (Roche), and QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Maedi-Visna Virus
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV36 100 FAM Cy5 gag polyprotein gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.