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Product Manual ViroReal® Kit MERS-CoV Download PDF
Material Safety Data Sheet ViroReal® Kit MERS-CoV Download PDF
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Background:  Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a beta coronavirus causing Middle East Respiratory Syndrome (MERS), a zoonotic and severe respiratory disease in humans. To date, MERS-CoV has only been isolated from dromedary camels and humans. In humans, it was first identified in April 2012. Camels are a likely primary source of the MERS-CoV infection in humans.

Description:  ViroReal® Kit MERS-CoV uses one-step reverse transcription RT real-time PCR for the detection of the nucleocapsid (N) gene of Middle East Respiratory Syndrome Coronavirus (MERS-CoV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® Kit MERS-CoV
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV03013 100 FAM Cy5 nucleocapsid (N) gene
DVEV03053 50 FAM Cy5 nucleocapsid (N) gene
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: N gene of MERS-CoV
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit MERS-CoV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® MERS-CoV
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV31 100 FAM Cy5 nucleocapsid (N) gene
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.