Background: Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a beta coronavirus causing Middle East Respiratory Syndrome (MERS), a zoonotic and severe respiratory disease in humans. To date, MERS-CoV has only been isolated from dromedary camels and humans. In humans, it was first identified in April 2012. Camels are a likely primary source of the MERS-CoV infection in humans.

Description: ViroReal® Kit MERS-CoV uses one-step reverse transcription RT real-time PCR for the detection of the nucleocapsid (N) gene of Middle East Respiratory Syndrome Coronavirus (MERS-CoV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well. For more detailed information see here.

ViroReal® Kit MERS-CoV
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEV03013100VIC/HEXCY5nucleocapsid (N) gene
DVEV0305350VIC/HEXCY5nucleocapsid (N) gene
For veterinary use only.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: N gene of MERS-CoV
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit MERS-CoV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® MERS-CoV
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV31100VIC/HEX-nucleocapsid (N) gene
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.