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Product Manual BactoReal® Kit Listeria monocytogenes Download PDF
Material Safety Data Sheet BactoReal® Kit Listeria monocytogenes Download PDF
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Background:  Listeria monocytogenes causes listeriosis an infectious but not contagious disease affecting primarily domestic animals, especially ruminants (cattle, sheep, goats) and is less common in human beings. Rarely the disease can be found in birds, horses and pigs as well as in rodents and fish. It can also occur in feral animals (e.g. game animals) as well as in poultry and other birds. Listeria are found ubiquitously in soil, water and in poorly made silage.

Description:  BactoReal® Kit Listeria monocytogenes uses real-time PCR for the detection of the hlyA gene of L. monocytogenes. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Listeria monocytogenes
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB00813 100 FAM Cy5 hlyA
DVEB00853 50 FAM Cy5 hlyA
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: hlyA gene of Listeria monocytogenes
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Listeria monocytogenes is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Listeria monocytogenes
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV1200 100 FAM Cy5 hlyA
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.