Background: Lawsonia intracellularis is a Gramnegative and obligate intracellular bacterium and the causative agent of porcine proliferative enteropathy (PPE), which is a transmissible enteric disease of growing pigs characterized by adenomatous proliferation of immature intestinal epithelial cells in the distal small intestines, particularly in the ileum. The infection is widely distributed among pig herds in many pig-producing countries, and affected pigs may exhibit impaired growth and diarrhoea.

Description: BactoReal® Kit Lawsonia intracellularis uses real-time PCR for the detection of the 16S rRNA gene of L. intracellularis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

BactoReal® Kit Lawsonia intracellularis
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEB01313100FAMCY516S rRNA
For veterinary use only.
Shipping Temperature: +4°C cool packs
Product Features:
  • Amplification and detection: 16S rRNA gene of Lawsonia intracellularis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Lawsonia intracellularis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Lawsonia intracellularis
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV300100FAM-16S rRNA
For veterinary use only.
Shipping Temperature: +4°C cool packs

Related Products: BactoReal®Lawsonia intracellularis Standard

BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.