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Description:  BactoReal® Kit Klebsiella uses real-time PCR for the detection of the hemolysin gene of Klebsiella pneumoniae, K. variicola, K. oxytoca and K. aerogenes. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Klebsiella
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB06713 100 FAM Cy5 hemolysin gene
DVEB06753 50 FAM Cy5 hemolysin gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C

PCR platforms:

BactoReal® Klebsiella wurde für folgende Geräte entwickelt und validiert: Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) und Mx3005P® QPCR System (Agilent), ist aber auch für andere real-time PCR Geräte geeignet.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Klebsiella
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV340 100 FAM Cy5 hemolysin gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

It detects DNA of Klebsiella species by targeting the hemolysin gene using real-time PCR.
The assay is validated for DNA extracted from tracheal swabs, washes, and lung tissue.
The artificial DNA IPC helps detect extraction issues and PCR inhibition. It is detected in the Cy5 channel.
The limit of detection is 9 DNA target copies per reaction (LoD95 = 9).
The kit includes all necessary reagents including PCR mix and water, while the assay includes only detection reagents.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.