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| Product Description EN BactoReal® Kit Klebsiella |  Download PDF |
| Product Manual EN BactoReal® Kit Klebsiella | Download PDF |
| Material Safety Data Sheet EN BactoReal® Kit Klebsiella | Download PDF |
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Background: Klebsiella is a Gram-negative, facultative anaerobic bacterium with a prominent polysaccharide capsule in the family Enterobacteriaceae. Members of this genus cause pneumonia and urogenital infections in carnivores and ungulates, mastitis in ruminants and pigs, enterocolitis in rabbits and sporadic septicaemia in a number of species.
Description: BactoReal® Kit Klebsiella uses real-time PCR for the detection of the hemolysin gene of Klebsiella pneumoniae, K. variicola, K. oxytoca and K. aerogenes. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.
| Ordernumber | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEB06713 | 100 | FAM | CY5 | hemolysin gene |
| DVEB06753 | 50 | FAM | CY5 | hemolysin gene |
| DVEB06711 | 100 | FAM | VIC | hemolysin gene |
| DVEB06751 | 50 | FAM | VIC | hemolysin gene |
Shipping Temperature: +4°C cool packs
- Amplification and detection: hemolysin gene of Klebsiella
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: BactoReal® Kit Klebsiella is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Ordernumber | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV340 | 100 | FAM | - | hemolysin gene |
Shipping Temperature: +4°C cool packs