Background: Histophilus somni is an unencapsulated gram-negative bacterium and a member of the Pasteurellaceae family. It is a common commensal in the lower reproductive and upper respiratory tract of cattle and other ruminants. H. somni is one of the bacterial agents responsible for bovine respiratory disease (BRD) complex and associated with respiratory disease, arthritis, mastitis, myocarditis and reproductive failure in sheep and cattle. Other important bacterial pathogens of BRD are Pasteurella multocida, Mannheimia haemolytica, Trueperella pyogenes and Mycoplasma bovis. Mixed infections with these organisms do occur.

Description: BactoReal® Kit Histophilus somni uses real-time PCR for the detection of the 16S rRNA gene of Histophilus somni. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

BactoReal® Kit Histophilus somni
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEB06113100FAMCY516S rRNA
For veterinary use only.
Shipping Temperature: +4°C cool packs
Product Features:
  • Amplification and detection: 16S rRNA gene of Histophilus somni
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Histophilus somni is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV333100FAM-16S rRNA
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.