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Download AllBackground: Histomonas meleagridis is a parasitic protozoan causing blackhead disease, infectious enterohepatitis, or histomoniasis. It can infect turkeys, chickens, and occasionally other galliform birds but is most deadly in turkeys. Chickens are more resistant to the effects of the infection but may act as carriers. Histomonas meleagridis induces extensive and severe necrosis of the tissues of the mucosa and submucosa of cecum and parenchyma of the liver. It is transmitted by the nematode Heterakis gallinarum or directly from the droppings of infected birds. Histomonas meleagridis interacts with other gut organisms, such as bacteria and coccidia, and depends on them for full virulence.
Description: ParoReal Kit Histomonas meleagridis uses real-time PCR for the detection of the 18S rRNA gene of H. meleagridis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.
| Ordernumber | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEP00311 | 100 | FAM | VIC/HEX | 18S rRNA |
| DVEP00313 | 100 | FAM | CY5 | 18S rRNA |
| DVEP00351 | 50 | FAM | VIC/HEX | 18S rRNA |
| DVEP00353 | 50 | FAM | CY5 | 18S rRNA |
Shipping Temperature: +4°C cool packs
- Amplification and detection: 18S rRNA gene of Histomonas meleagridis
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: ParoReal Kit Histomonas meleagridis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Ordernumber | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV267 | 100 | FAM | - | 18S rRNA |
Shipping Temperature: +4°C cool packs
Related Products: ParoRealHistomonas meleagridis Standard