Background: Escherichia coli is a Gramnegative bacterium that is a member of the normal gut flora of humans and animals. Most E. coli strains are harmless, but some serotypes carry virulence factors, which enable them to be pathogenic in target hosts. These pathogenic E. coli are implicated in a number of diseases including diarrhoea, septicaemia and urinary tract infections in humans and animals. The dxs gene (1-deoxyxylulose-5-phosphate synthase gene) can be found in E. coli and Shigella strains.

Description: BactoReal® Kit Escherichia coli uses real-time PCR for the detection of the dxs gene of E. coli and Shigella. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

BactoReal® Kit Escherichia coli
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEB01911100FAMVIC/HEXdxs
DVEB01913100FAMCY5dxs
DVEB0195150FAMVIC/HEXdxs
DVEB0195350FAMCY5dxs
For veterinary use only.
Shipping Temperature: +4°C cool packs
Product Features:
  • Amplification and detection: dxs gene of Escherichia coli
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Escherichia coli is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.


Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Escherichia coli
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV914100FAM-dxs
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.