Background: The genus Enterococcus includes more than 17 species and until 1984, Enterococcus species were classified as Group D Streptococcus. This genus belongs to lactic acid bacteria of the phylum Firmicutes. Enterococci are Grampositive cocci, they are facultative anaerobic organisms that can survive and grow in many environments. They are part of the normal intestinal flora of humans and animals but also found in soil, water, plants and insects. Enterococci are important pathogens responsible for serious infections. In cattle, enterococci have been associated with diarrhea in calves and bovine mastitis in dairy cattle.

Description: BactoReal® Kit Enterococcus spp. uses real-time PCR for the detection of the 23S rRNA gene of species of the genus Enterococcus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

BactoReal® Kit Enterococcus spp.
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEB03411100FAMVIC/HEX23S rRNA
DVEB03413100FAMCY523S rRNA
DVEB0345150FAMVIC/HEX23S rRNA
DVEB0345350FAMCY523S rRNA
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: +4°C cool packs
Product Features:
  • Amplification and detection: 23S rRNA gene of Enterococcus spp.
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Enterococcus spp. is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.


Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Enterococcus spp.
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV905100FAM-23S rRNA
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.