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Product Manual ParoReal® Echinococcus multilocularis Download PDF
Material Safety Data Sheet ParoReal® Echinococcus multilocularis Download PDF
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Background:  Echinococcus multilocularis is a cyclophyllid tapeworm causing echinococcosis, a disease that affects many canid species including wolves, coyotes, foxes, jackals and domesticated dogs. Humans can also contract this disease. Echinococcus multilocularis requires two types of hosts to mature properly. It is transmitted to intermediate hosts via the ingestion of eggs in infected food. The eggs travel to the lungs, liver, and other internal organs where they develop into multilocular cysts. Finally, Echinococcus multilocularis is transmitted to definitive hosts by means of eating infected, cyst-containing organs. There it becomes attached to the tissues and grows.

Description:  ParoReal® Kit Echinococcus multilocularis uses real-time PCR for the detection of the mitochondrial ND1 gene of E. multilocularis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

ParoReal® Kit Echinococcus multilocularis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEP00113 100 FAM Cy5 mitochondrial ND1
DVEP00153 50 FAM Cy5 mitochondrial ND1
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: ND1 gene of Echinococcus multilocularis
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms

PCR platforms: ParoReal® Kit Echinococcus multilocularis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ParoReal® Echinococcus multilocularis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV266 100 FAM Cy5 mitochondrial ND1
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

It detects DNA of Echinococcus multilocularis by targeting the mitochondrial ND1 gene via real-time PCR.
The assay is validated for DNA extracted from biopsy tissue.
An artificial DNA IPC is added during extraction and detected in the Cy5 channel to monitor extraction quality and PCR inhibition.
The assay detects as few as 5 target copies per PCR reaction.
The assay includes detection reagents only, while the kit includes all necessary reagents including PCR mix and water.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.