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Product Manual ParoReal® Kit Echinococcus multilocularis Download PDF
Material Safety Data Sheet ParoReal® Kit Echinococcus multilocularis Download PDF
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Background:  Echinococcus multilocularis is a cyclophyllid tapeworm causing echinococcosis, a disease that affects many canid species including wolves, coyotes, foxes, jackals and domesticated dogs. Humans can also contract this disease. Echinococcus multilocularis requires two types of hosts to mature properly. It is transmitted to intermediate hosts via the ingestion of eggs in infected food. The eggs travel to the lungs, liver, and other internal organs where they develop into multilocular cysts. Finally, Echinococcus multilocularis is transmitted to definitive hosts by means of eating infected, cyst-containing organs. There it becomes attached to the tissues and grows.

Description:  ParoReal® Kit Echinococcus multilocularis uses real-time PCR for the detection of the mitochondrial ND1 gene of E. multilocularis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

ParoReal® Kit Echinococcus multilocularis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEP00113 100 FAM Cy5 mitochondrial ND1
DVEP00153 50 FAM Cy5 mitochondrial ND1
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: ND1 gene of Echinococcus multilocularis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ParoReal® Kit Echinococcus multilocularis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ParoReal® Echinococcus multilocularis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV266 100 FAM Cy5 mitochondrial ND1
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.