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Product Manual ViroReal® EMCV (Encephalomyocarditis-Virus) Download PDF
Material Safety Data Sheet ViroReal® EMCV (Encephalomyocarditis-Virus) Download PDF
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Background:  Encephalomyocarditis virus (EMCV) is one of three virus species in the genus Cardiovirus in the family Picornaviridae and is a single stranded RNA (ssRNA) virus that causes encephalomyocarditis and reproductive disease in swine and zoologic mammals. Infected piglets suffer from encephalitis, myocarditis and sudden death. Reproductive problems include poor conception rates, embryo resorption, mummification, stillbirths, abortions and neonatal death. The disease affects the nervous, reproductive, respiratory and circulatory system of pigs. Fatal myocarditis due to EMC virus infection has been seen in primates, even-and odd-toed ungulates, elephants, carnivores and rodents. The disease can be found worldwide. The most important mode of virus transmission seems to be rodent-to-pig transmission, and rodents act as the major reservoir of the disease. Rats and other rodents probably infect pigs directly or through diseased carcasses and contaminated feeds or water supplies.

Description:  ViroReal® Kit EMCV uses one-step reverse transcription RT real-time PCR for the detection of the 3´UTR RNA of EMCV. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® Kit EMCV (Encephalomyocarditis-Virus)
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV01013 100 FAM Cy5 3´UTR RNA
DVEV01053 50 FAM Cy5 3´UTR RNA
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: 3´UTR of EMCV
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms

PCR platforms: ViroReal® Kit EMCV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® EMCV (Encephalomyocarditis-Virus)
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV30 100 FAM Cy5 3´UTR RNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.