Background: Encephalomyocarditis virus (EMCV) is one of three virus species in the genus Cardiovirus in the family Picornaviridae and is a single stranded RNA (ssRNA) virus that causes encephalomyocarditis and reproductive disease in swine and zoologic mammals. Infected piglets suffer from encephalitis, myocarditis and sudden death. Reproductive problems include poor conception rates, embryo resorption, mummification, stillbirths, abortions and neonatal death. The disease affects the nervous, reproductive, respiratory and circulatory system of pigs. Fatal myocarditis due to EMC virus infection has been seen in primates, even-and odd-toed ungulates, elephants, carnivores and rodents. The disease can be found worldwide. The most important mode of virus transmission seems to be rodent-to-pig transmission, and rodents act as the major reservoir of the disease. Rats and other rodents probably infect pigs directly or through diseased carcasses and contaminated feeds or water supplies.

Description: ViroReal® Kit EMCV uses one-step reverse transcription RT real-time PCR for the detection of the 3´UTR RNA of EMCV. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well. For more detailed information see here.

ViroReal® Kit EMCV
OrdernumberReactionsChannel PathogenChannel IPCTarget
For veterinary use only.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: 3´UTR of EMCV
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit EMCV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® EMCV
OrdernumberReactionsChannel PathogenChannel IPCTarget
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.