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Product Manual ParoReal® Kit Cryptosporidium parvum Download PDF
Material Safety Data Sheet ParoReal® Kit Cryptosporidium parvum Download PDF
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Background:  Cryptosporidium parvum is one of several protozoal species that cause cryptosporidiosis, a parasitic disease of the mammalian intestinal tract.It is a zoonotic and obligate intracellular parasite. Primary symptoms of C. parvum infection are acute, watery, and non-bloody diarrhea. Extra-intestinal sites include the lung, liver and gall bladder where it causes respiratory cryptosporidosis, hepatitis and cholecystitis. Infection is caused by ingestion of sporulated oocysts transmitted by the faecal-oral route.

Description:  ParoReal® Kit Cryptosporidium parvum uses real-time PCR for the detection of the GP60 gene of Cryptosporidium parvum. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

ParoReal® Kit Cryptosporidium parvum
Order Number Reactions Channel Pathogen Channel IPC Target
DVEP00613 100 FAM Cy5 GP60
DVEP00653 50 FAM Cy5 GP60
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: GP60 gene of Cryptosporidium parvum
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ParoReal® Kit Cryptosporidium parvum is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ParoReal® Cryptosporidium parvum
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV270 100 FAM Cy5 GP60
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.