Background:  Corynebacterium pseudotuberculosis is a Grampositive bacterium and the aetiological agent of external or cutaneous abscessation, lymphangitis, and internal abscessation affecting sheep, goats, horses, cattle and rarely humans as well. Corynebacterium pseudotuberculosis produces an exotoxin called phospholipase D that enhances dissemination of the bacteria by damaging endothelial cells and increasing vascular permeability.

Description:  BactoReal® Kit Corynebacterium pseudotuberculosis uses real-time PCR for the detection of the phosholipase D (pld) gene of Corynebacterium pseudotuberculosis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Corynebacterium pseudotuberculosis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB05213 100 FAM Cy5 pld
DVEB05253 50 FAM Cy5 pld
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: pld gene of Corynebacterium pseudotuberculosis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Corynebacterium pseudotuberculosis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Corynebacterium pseudotuberculosis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV919 100 FAM Cy5 pld
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.