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Download AllBackground: Clostridium perfringens is a spore-forming Grampositive bacterium present in many environmental sources as well as in the intestines of humans and animals. Under certain predisposing conditions, Clostridium perfringens proliferates rapidly, produces toxins and causes disease such as gas gangrene, necrotic enteritis and enterotoxemia. This bacterium is an important cause of food poisoning. Stress and the feed composition are believed to be of major importance for the development of disease. Clostridium perfringens strains are classified into five types (A–E) based on their ability to produce the major lethal toxins alpha (cpa), beta (cpb), epsilon (etx), and iota (iap) which are responsible for the cytotoxic and necrotic activity associated with infection. In addition, strains of C. perfringens may also produce a number of other toxins, including enterotoxin (cpe), beta-2 toxin (cpb2) or theta toxin.
Description: BactoReal® Kit Clostridium perfringens uses real-time PCR for the detection of the 16S rRNA gene of all five serotypes of C. perfringens. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.
| Ordernumber | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEB06511 | 100 | FAM | VIC/HEX | 16S |
| DVEB06513 | 100 | FAM | CY5 | 16S |
| DVEB06551 | 50 | FAM | VIC/HEX | 16S |
| DVEB06553 | 50 | FAM | CY5 | 16S |
Shipping Temperature: +4°C cool packs
- Amplification and detection: 16S rRNA gene of Clostridium perfringens
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: BactoReal® Kit Clostridium perfringens is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Ordernumber | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV928 | 100 | FAM | - | 16S |
Shipping Temperature: +4°C cool packs