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Download AllBackground: Clostridium difficile is a Grampositive spore-forming anaerobic bacterium, commonly found in low concentrations in the intestine of clinically normal animals and humans. In higher concentration Clostridium difficile is an important cause of diarrhea in neonatal swine. Affected piglets may have dyspnea, abdominal distention, and scrotal edema. Diarrhea may not be present in all pigs affected. Pathogenic C. difficile strains produce multiple toxins. The best characterized are enterotoxin (Clostridium difficile toxin A causing fluid secretion into the gut lumen) and cytotoxin (Clostridium difficile toxin B), both of which may produce diarrhea and inflammation.
Description: BactoReal® Kit Clostridium difficile uses real-time PCR for the detection of the Clostridium difficile toxin A (tcdA) gene and Clostridium difficile toxin B (tcdB) gene of C. difficile. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.
| Ordernumber | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEB04213 | 100 | FAM & VIC/HEX | CY5 | tcdA/tcdB |
| DVEB04253 | 50 | FAM & VIC/HEX | CY5 | tcdA/tcdB |
Shipping Temperature: +4°C cool packs
- Amplification and detection: tcdA gene and tcdB gene of Clostridium difficile
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: BactoReal® Kit Clostridium difficile is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Ordernumber | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV920 | 100 | FAM & VIC/HEX | - | tcdA/tcdB |
Shipping Temperature: +4°C cool packs