Background: The Chlamydiaceae family currently includes two genera and one candidate genus: genus Chlamydia (including the species Chlamydia muridarum, Chlamydia suis, Chlamydia trachomatis), genus Chlamydophila (including the species Chlamydophila abortus, Chlamydophila caviae Chlamydophila felis, Chlamydia pecorum, Chlamydophila pneumoniae, Chlamydophila psittaci), and candidatus Clavochlamydia. All Chlamydiaceae are obligate intracellular Gramnegative bacteria that cause diseases in humans and animals worldwide.

Description: BactoReal® Kit Chlamydiaceae uses real-time PCR for the detection of the 23S rRNA gene of C. muridarum, C. suis, C. trachomatis, C. abortus, C. caviae, C. felis, C. pecorum, C. pneumoniae and C. psittaci. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG) to minimize PCR cross-contamination. For more detailed information see here.

BactoReal® Kit Chlamydiaceae
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEB03113100FAMCY523S rRNA
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: +4°C cool packs
Product Features:
  • Amplification and detection: 23S rRNA gene of Chlamydiaceae
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Chlamydiaceae is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Chlamydiaceae
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV1400100FAM-23S rRNA
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.