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| Product Description EN ViroReal® Kit CAEV |  Download PDF |
| Product Manual EN ViroReal® Kit CAEV | Download PDF |
| Material Safety Data Sheet EN ViroReal® Kit CAEV | Download PDF |
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Background: The caprine arthritis - encephalitis virus (CAEV) of goats is an enveloped, single-stranded RNA small ruminant lentivirus (SRLV) in the family Retroviridae. There are several, genetically distinct isolates of the virus that differ in virulence. The CAE-virus is closely related to the Maedi-Visna-virus (MVV) of sheep. Both infect cells of the monocyte/macrophage lineage and cause lifelong infections. Transmission of the CAEV is through ingestion of virus-infected goat milk but also through virus-infected secretion of affected animals and droplet infection. Clinical symptoms such as severe arthritis of the carpal joint are seen in approximately 20 % of infected goats. Subclinical or clinical interstitial pneumonia, mastitis with lower milk production and chronic wasting have also been attributed to infection with the virus.
Description: ViroReal® Kit CAEV uses one-step reverse transcription RT real-time PCR for the detection of the gag polyprotein gene of Caprine Arthritis and Encephalitis Virus (CAEV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well. For more detailed information see here.
| Ordernumber | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEV03411 | 100 | FAM | VIC/HEX | gag polyprotein gene |
| DVEV03413 | 100 | FAM | CY5 | gag polyprotein gene |
| DVEV03451 | 50 | FAM | VIC/HEX | gag polyprotein gene |
| DVEV03453 | 50 | FAM | CY5 | gag polyprotein gene |
Shipping Temperature: -20°C dry ice
- Amplification and detection: gag gene of CAEV
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle RT-PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: ViroReal® Kit CAEV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Ordernumber | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV35 | 100 | FAM | - | gag polyprotein gene |
Shipping Temperature: +4°C cool packs