Background:  The caprine arthritis - encephalitis virus (CAEV) of goats is an enveloped, single-stranded RNA small ruminant lentivirus (SRLV) in the family Retroviridae. There are several, genetically distinct isolates of the virus that differ in virulence. The CAE-virus is closely related to the Maedi-Visna-virus (MVV) of sheep. Both infect cells of the monocyte/macrophage lineage and cause lifelong infections. Transmission of the CAEV is through ingestion of virus-infected goat milk but also through virus-infected secretion of affected animals and droplet infection. Clinical symptoms such as severe arthritis of the carpal joint are seen in approximately 20 % of infected goats. Subclinical or clinical interstitial pneumonia, mastitis with lower milk production and chronic wasting have also been attributed to infection with the virus.

Description:  ViroReal® Kit CAEV uses one-step reverse transcription RT real-time PCR for the detection of the gag polyprotein gene of Caprine Arthritis and Encephalitis Virus (CAEV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® CAEV
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV03413 100 FAM Cy5 gag polyprotein gene
DVEV03453 50 FAM Cy5 gag polyprotein gene
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: gag gene of CAEV
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit CAEV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® CAEV
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV35 100 FAM Cy5 gag polyprotein gene
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.