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Product Manual ViroReal® Bovine Torovirus Download PDF
Material Safety Data Sheet ViroReal® Bovine Torovirus Download PDF
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Background:  The species bovine torovirus (BToV) is a member of the family Coronaviridae, subfamily Torovirinae. The genome is a single-stranded, positive-sense RNA molecule. The kidney-shaped BToV causes diarrhea in calves but respiratory infections have also been reported and it has a worldwide distribution. Bovine torovirus, formerly called breda virus (BRV), was originally isolated from diarrheic calves in Breda, Iowa, in 1979.

Description:  ViroReal® Kit Bovine Torovirus uses one-step reverse transcription RT real-time PCR for the detection of the membrane protein (M) gene of the BToV. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® Kit Bovine Torovirus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV01613 100 FAM Cy5 membrane protein M gene
DVEV01653 50 FAM Cy5 membrane protein M gene
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: M gene of Bovine Torovirus
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: ViroReal® Kit Bovine Torovirus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Bovine Torovirus
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV90 100 FAM Cy5 membrane protein M gene
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.