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Product Manual ViroReal® Bovine Torovirus Download PDF
Material Safety Data Sheet ViroReal® Bovine Torovirus Download PDF
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Background:  The species bovine torovirus (BToV) is a member of the family Coronaviridae, subfamily Torovirinae. The genome is a single-stranded, positive-sense RNA molecule. The kidney-shaped BToV causes diarrhea in calves but respiratory infections have also been reported and it has a worldwide distribution. Bovine torovirus, formerly called breda virus (BRV), was originally isolated from diarrheic calves in Breda, Iowa, in 1979.

Description:  ViroReal® Kit Bovine Torovirus uses one-step reverse transcription RT real-time PCR for the detection of the membrane protein (M) gene of the BToV. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® Kit Bovine Torovirus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV01613 100 FAM Cy5 membrane protein M gene
DVEV01653 50 FAM Cy5 membrane protein M gene
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: M gene of Bovine Torovirus
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: ViroReal® Kit Bovine Torovirus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Bovine Torovirus
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV90 100 FAM Cy5 membrane protein M gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The assay detects RNA of Bovine Torovirus (BToV) by targeting the membrane protein (M) gene using real-time RT-PCR.
BToV is associated with diarrhea in calves and has been reported worldwide. Respiratory infections have also been observed.
An artificial RNA internal control (IPC) is added during extraction and detected in the Cy5 channel to monitor inhibition and reagent integrity.
The assay has an analytical sensitivity of approximately 50–100 RNA copies per PCR reaction.
An assay provides only the detection reagents, while a kit includes additional components such as PCR mix and controls.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.