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Product Manual ViroReal® Kit Bovine Nebovirus Download PDF
Material Safety Data Sheet ViroReal® Kit Bovine Nebovirus Download PDF
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Background:  Neboviruses are non-enveloped, single-stranded, linear, positive-sense RNA viruses ((+)ssRNA), belonging to the Caliciviridae family. The only currently known species in the genus Nebovirus is newbury-1 virus. Cattle, especially calves, serve as natural hosts. Neboviral infection is associated with gastrointestinal disorders and necrotizing hepatitis leading to fatal hemorrhages in calves. The disease often remains endemic due to the lack of a commercially available vaccine.

Description:  ViroReal® Kit Nebovirus uses one-step reverse transcription RT real-time PCR for the detection of ORF 1 of bovine nebovirus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® Kit Bovine Nebovirus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV02713 100 FAM Cy5 ORF 1
DVEV02753 50 FAM Cy5 ORF 1
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: ORF 1 of Nebovirus
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit Nebovirus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Bovine Nebovirus
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV23 100 FAM Cy5 ORF 1
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.