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Download AllBackground: Neboviruses are non-enveloped, single-stranded, linear, positive-sense RNA viruses ((+)ssRNA), belonging to the Caliciviridae family. The only currently known species in the genus Nebovirus is newbury-1 virus. Cattle, especially calves, serve as natural hosts. Neboviral infection is associated with gastrointestinal disorders and necrotizing hepatitis leading to fatal hemorrhages in calves. The disease often remains endemic due to the lack of a commercially available vaccine.
Description: ViroReal® Kit Nebovirus uses one-step reverse transcription RT real-time PCR for the detection of ORF 1 of bovine nebovirus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well. For more detailed information see here.
| Ordernumber | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEV02711 | 100 | FAM | VIC/HEX | ORF 1 |
| DVEV02713 | 100 | FAM | CY5 | ORF 1 |
| DVEV02751 | 50 | FAM | VIC/HEX | ORF 1 |
| DVEV02753 | 50 | FAM | CY5 | ORF 1 |
Shipping Temperature: -20°C dry ice
- Amplification and detection: ORF 1 of Nebovirus
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle RT-PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: ViroReal® Kit Nebovirus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Ordernumber | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV23 | 100 | FAM | - | ORF 1 |
Shipping Temperature: +4°C cool packs