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Product Manual ViroReal® Bovine Herpesvirus I Download PDF
Material Safety Data Sheet ViroReal® Bovine Herpesvirus I Download PDF
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Background:  Bovine herpesvirus 1 (BHV-1) is a DNA virus of the family Herpesviridae, subfamily Alphaherpesvirinae. BHV-1 can cause both clinical and subclinical infections in cattle, including infectious bovine rhinotracheitis (IBR), infectious pustular vulvovaginitis (IBV) and infectious balanoposthitis (IBP). BHV-1 causes a lifelong latent infection in the trigeminal or sacral ganglia. Animals with a latent BHV-1 infection may serve as a source of infection for susceptible animals. BHV-1 is transmitted mainly through respiratory, ocular or genital secretions.

Description:  ViroReal® Kit Bovine Herpesvirus 1 is a real-time PCR for the qualitative detection of the DNA of the UL27 gene of Bovine Herpesvirus 1 (BHV-1). Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit Bovine Herpesvirus I
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV02913 100 FAM Cy5 UL27
DVEV02953 50 FAM Cy5 UL27
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: DNA of the UL27 gene of Bovine Herpesvirus 1
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit Bovine Herpesvirus 1 was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent) and LightCycler® 480 II (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), cobas z 480 Analyzer (Roche), and QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Bovine Herpesvirus I
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV28 100 FAM Cy5 UL27
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The assay qualitatively detects DNA of Bovine Herpesvirus 1 (BHV-1) by targeting the UL27 gene using real-time PCR.
The assay is validated for DNA extracted from tissue and swab samples of cattle.
An artificial DNA IPC is added during extraction and detected in the Cy5 channel to monitor DNA extraction and identify PCR inhibition.
The assay reliably detects at least 10 DNA target copies per reaction and shows linearity from 100 to 1,000,000 copies.
The assay includes only detection reagents and requires an external PCR mix, while the kit includes a complete reagent set including PCR mix and water.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.