Background: Bovine coronavirus (BCoV) is a member of the genus Coronavirus (Betacoronavirus), family Coronaviridae. The BCoV virion is enveloped and spherical in shape. The genome is a single-stranded, positive-sense RNA molecule. BCoV causes diarrhea in adult cattle (Winter Dysentery) and wild ruminants, as well as enteric and respiratory diseases in calves. Winter Dysentery is characterized by high morbidity (50-100%) and low mortality (1-2%). The highest incidence is between November and April when cattle are kept in close confinement. In calves, morbidity and mortality are high. BCoV has been found in cattle worldwide.

Description: ViroReal® Kit Bovine Coronavirus uses one-step reverse transcription RT real-time PCR for the detection of the nucleocapsid protein gene (N gene) of the BCoV. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well. For more detailed information see here.

ViroReal® Kit Bovine Coronavirus
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEV00911100FAMVIC/HEXnucleocapsid protein gene
DVEV00913100FAMCY5nucleocapsid protein gene
DVEV0095150FAMVIC/HEXnucleocapsid protein gene
DVEV0095350FAMCY5nucleocapsid protein gene
For veterinary use only.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: N gene of Bovine Coronavirus
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit Bovine Coronavirus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Bovine Coronavirus
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV20100FAM-nucleocapsid protein gene
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.