Background:  Bordetella bronchiseptica is a Gram-negative bacterium found as a normal inhabitant of the respiratory tract in a variety of animals such as dogs, pigs, some rodents, as well as humans. In some cases, it causes pneumonia, canine infectious tracheobronchitis (kennel cough), bronchopneumonia in cats, and atrophic rhinitis in pigs. It is widely distributed in the swine population. Here, some strains cause a mild and non-progressive rhinitis that heals spontaneously. However if toxigenic pasteurella are present in the herd then a combination of the two organisms can produce severe progressive rhinitis (PAR). Bordetella bronchiseptica can also be a secondary opportunist invader in pneumonia.

Description:  BactoReal® Kit Bordetella bronchiseptica uses real-time PCR for the detection of the bfrA gene of Bordetella bronchiseptica. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Bordetella bronchiseptica
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB02413 100 FAM Cy5 bfrA gene
DVEB02453 50 FAM Cy5 bfrA gene
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: bfrA gene of Bordetella bronchiseptica
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Bordetella bronchiseptica is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Bordetella bronchiseptica
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV1250 100 FAM Cy5 bfrA gene
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.