Background:  Bluetongue virus (BTV) is a segmented, linear, double-stranded RNA virus (dsRNA), belonging to the Reoviridae family and is divided into 28 serotypes. The virus is transmitted by biting midges and infects wild and domestic ruminants such as sheep and less frequently goats, deer and cattle. There is no appropriate treatment of acute disease, which is associated with high morbidity and mortality in some sheep breeds. Prophylactic immunisation with attenuated and inactivated vaccines is available. However, they do not cover all virulent BTV serotypes.

Description:  ViroReal® Kit Bluetongue Virus (BTV) uses one-step reverse transcription RT real-time PCR for the detection of the segment 10 (NS3) of BTV. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® Bluetongue Virus (BTV)
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV02213 100 FAM Cy5 NS3
DVEV02253 50 FAM Cy5 NS3
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: NS3 gene of Bluetongue Virus (BTV)
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit Bluetongue Virus (BTV) is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Bluetongue Virus (BTV)
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV22 100 FAM Cy5 NS3
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.