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Product Manual ViroReal® Kit BRSV Download PDF
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Background:  Bovine respiratory syncytial virus (BRSV) is an enveloped, non-segmented, single-stranded negative sense ((-)ssRNA) RNA virus that causes respiratory tract infections in cattle. It is an important virus in the bovine respiratory disease complex, affecting mostly young beef and dairy cattle. In stressful periods such as transportation or if co-infections are present (which is often the case in RSV infections), severe forms of pneumonia may develop. Treatment of co-infections with antibiotics, as well as disease control measurements are indicated.

Description:  ViroReal® Kit BRSV uses one-step reverse transcription RT real-time PCR for the detection of the nucleoprotein (N) gene of bovine respiratory syncytial virus (BRSV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® Kit BRSV
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV02413 100 FAM Cy5 nucleoprotein N gene
DVEV02453 50 FAM Cy5 nucleoprotein N gene
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: N gene of BRSV
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit BRSV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Kit BRSV
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV25 100 FAM Cy5 nucleoprotein N gene
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.